Light-sheet microscopy using an Airy beam

T Vettenburg, HIC Dalgarno, J Nylk, C Coll-Lladó… - Nature …, 2014 - nature.com
T Vettenburg, HIC Dalgarno, J Nylk, C Coll-Lladó, DEK Ferrier, T Čižmár, FJ Gunn-Moore
Nature methods, 2014nature.com
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal
sample exposure. However, the rapid divergence of a traditional Gaussian light sheet
restricts the field of view (FOV) that provides innate subcellular resolution. We show that the
Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast
to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic
asymmetric excitation pattern results in all fluorescence contributing positively to the …
Abstract
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
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