A line scanned light-sheet microscope with phase shaped self-reconstructing beams

FO Fahrbach, A Rohrbach - Optics express, 2010 - opg.optica.org
FO Fahrbach, A Rohrbach
Optics express, 2010opg.optica.org
We recently demonstrated that Microscopy with Self-Reconstructing Beams (MISERB)
increases both image quality and penetration depth of illumination beams in strongly
scattering media. Based on the concept of line scanned light-sheet microscopy, we present
an add-on module to a standard inverted microscope using a scanned beam that is shaped
in phase and amplitude by a spatial light modulator. We explain technical details of the
setup as well as of the holograms for the creation, positioning and scaling of static light …
We recently demonstrated that Microscopy with Self-Reconstructing Beams (MISERB) increases both image quality and penetration depth of illumination beams in strongly scattering media. Based on the concept of line scanned light-sheet microscopy, we present an add-on module to a standard inverted microscope using a scanned beam that is shaped in phase and amplitude by a spatial light modulator. We explain technical details of the setup as well as of the holograms for the creation, positioning and scaling of static light-sheets, Gaussian beams and Bessel beams. The comparison of images from identical sample areas illuminated by different beams allows a precise assessment of the interconnection between beam shape and image quality. The superior propagation ability of Bessel beams through inhomogeneous media is demonstrated by measurements on various scattering media.
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