Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope
Optics express, 2016•opg.optica.org
A methodology for the adaptive control and correction of phase aberrations in the
illumination arm of a light-sheet fluorescence microscope has been developed. The method
uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the
sample. Using this signal, the aberrations in the illumination arm are subsequently corrected
with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in
significant improvement in the axial resolution, has been demonstrated by imaging Tg (fli …
illumination arm of a light-sheet fluorescence microscope has been developed. The method
uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the
sample. Using this signal, the aberrations in the illumination arm are subsequently corrected
with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in
significant improvement in the axial resolution, has been demonstrated by imaging Tg (fli …
A methodology for the adaptive control and correction of phase aberrations in the illumination arm of a light-sheet fluorescence microscope has been developed. The method uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the sample. Using this signal, the aberrations in the illumination arm are subsequently corrected with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in significant improvement in the axial resolution, has been demonstrated by imaging Tg(fli:GFP) zebrafish embryos.
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