Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
Nature methods, 2014•nature.com
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major
challenge in neuroscience. Here we demonstrate simultaneous functional imaging of
neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in
larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes
of∼ 700 μm× 700 μm× 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-
speed volumetric calcium imaging.
challenge in neuroscience. Here we demonstrate simultaneous functional imaging of
neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in
larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes
of∼ 700 μm× 700 μm× 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-
speed volumetric calcium imaging.
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
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