FoxM1 is required for execution of the mitotic programme and chromosome stability

J Laoukili, MRH Kooistra, A Brás, J Kauw… - Nature cell …, 2005 - nature.com
J Laoukili, MRH Kooistra, A Brás, J Kauw, RM Kerkhoven, A Morrison, H Clevers
Nature cell biology, 2005nature.com
Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of
subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1
regulates expression of many G2-specific genes and is essential for chromosome stability.
Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome
mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of
cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target …
Abstract
Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1 regulates expression of many G2-specific genes and is essential for chromosome stability. Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target of FoxM1 identified here, is essential for precise functioning of the mitotic spindle checkpoint. Thus, our data uncover a transcriptional cluster regulated by FoxM1 that is essential for proper mitotic progression.
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