[HTML][HTML] Lupus and proliferative nephritis are PAD4 independent in murine models

RA Gordon, JM Herter, F Rosetti, AM Campbell… - JCI insight, 2017 - ncbi.nlm.nih.gov
RA Gordon, JM Herter, F Rosetti, AM Campbell, H Nishi, M Kashgarian, SI Bastacky…
JCI insight, 2017ncbi.nlm.nih.gov
Though recent reports suggest that neutrophil extracellular traps (NETs) are a source of
antigenic nucleic acids in systemic lupus erythematosus (SLE), we recently showed that
inhibition of NETs by targeting the NADPH oxidase complex via cytochrome b-245, β
polypeptide (cybb) deletion exacerbated disease in the MRL. Fas lpr lupus mouse model.
While these data challenge the paradigm that NETs promote lupus, it is conceivable that
global regulatory properties of cybb and cybb-independent NETs confound these findings …
Abstract
Though recent reports suggest that neutrophil extracellular traps (NETs) are a source of antigenic nucleic acids in systemic lupus erythematosus (SLE), we recently showed that inhibition of NETs by targeting the NADPH oxidase complex via cytochrome b-245, β polypeptide (cybb) deletion exacerbated disease in the MRL. Fas lpr lupus mouse model. While these data challenge the paradigm that NETs promote lupus, it is conceivable that global regulatory properties of cybb and cybb-independent NETs confound these findings. Furthermore, recent reports indicate that inhibitors of peptidyl arginine deiminase, type IV (Padi4), a distal mediator of NET formation, improve lupus in murine models. Here, to clarify the contribution of NETs to SLE, we employed a genetic approach to delete Padi4 in the MRL. Fas lpr model and used a pharmacological approach to inhibit PADs in both the anti–glomerular basement membrane model of proliferative nephritis and a human-serum-transfer model of SLE. In contrast to prior inhibitor studies, we found that deletion of Padi4 did not ameliorate any aspect of nephritis, loss of tolerance, or immune activation. Pharmacological inhibition of PAD activity had no effect on end-organ damage in inducible models of glomerulonephritis. These data provide a direct challenge to the concept that NETs promote autoimmunity and target organ injury in SLE.
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