Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling machines
Journal of proteome research, 2006•ACS Publications
We use affinity purification of the double bromodomain protein Brd2 to isolate a
multicomponent nuclear complex from cultured cells, and apply mass spectrometry/
proteomics methods to identify the participants. We then confirm by immunoblot several
transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling
complex, which regulate transcription control of cyclin A. This multiprotein complex is likely
to contribute to cell cycle control and play a role in proliferation and cancer. Keywords …
multicomponent nuclear complex from cultured cells, and apply mass spectrometry/
proteomics methods to identify the participants. We then confirm by immunoblot several
transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling
complex, which regulate transcription control of cyclin A. This multiprotein complex is likely
to contribute to cell cycle control and play a role in proliferation and cancer. Keywords …
We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.
Keywords: bromodomain • multiprotein complexes • affinity chromatography • high pressure liquid chromatography • mass spectral analysis • transcription factors • TATA binding protein-associated factors • chromatin assembly and disassembly • cyclin A
ACS Publications