[HTML][HTML] Characterization of the Receptor Binding Sites of Human Leukemia Inhibitory Factor and Creation of Antagonists (∗)
KR Hudson, AB Vernallis, JK Heath - Journal of Biological Chemistry, 1996 - ASBMB
Residues in human leukemia inhibitory factor (hLIF) crucial for binding to both the human
LIF receptor (R) and gp130 were identified by analysis of alanine scanning mutants of hLIF
in assays for both receptor binding and bioactivity. The region of hLIF most important for
binding to the hLIF-R is composed of residues from the amino terminus of the D-helix,
carboxyl terminus of the B-helix, and CD loop. This site forms a distinct surface at the end of
the four-helix bundle in the tertiary structure of the closely related murine LIF. The two …
LIF receptor (R) and gp130 were identified by analysis of alanine scanning mutants of hLIF
in assays for both receptor binding and bioactivity. The region of hLIF most important for
binding to the hLIF-R is composed of residues from the amino terminus of the D-helix,
carboxyl terminus of the B-helix, and CD loop. This site forms a distinct surface at the end of
the four-helix bundle in the tertiary structure of the closely related murine LIF. The two …