Versican V2 is a major extracellular matrix component of the mature bovine brain
M Schmalfeldt, MT Dours-Zimmermann… - Journal of Biological …, 1998 - jbc.org
We have isolated and characterized the proteoglycan isoforms of versican from bovine brain
extracts. Our approach included (i) cDNA cloning and sequencing of the entire open reading
frame encoding the bovine versican splice variants;(ii) preparation of antibodies against
bovine versican using recombinant core protein fragments and synthetic peptides;(iii)
isolation of versican isoforms by ammonium sulfate precipitation followed by anion
exchange and hyaluronan affinity chromatography; and (iv) characterization by SDS …
extracts. Our approach included (i) cDNA cloning and sequencing of the entire open reading
frame encoding the bovine versican splice variants;(ii) preparation of antibodies against
bovine versican using recombinant core protein fragments and synthetic peptides;(iii)
isolation of versican isoforms by ammonium sulfate precipitation followed by anion
exchange and hyaluronan affinity chromatography; and (iv) characterization by SDS …