Three-dimensional structure of canine adenovirus serotype 2 capsid

G Schoehn, M El Bakkouri, CMS Fabry, O Billet… - Journal of …, 2008 - Am Soc Microbiol
G Schoehn, M El Bakkouri, CMS Fabry, O Billet, LF Estrozi, L Le, DT Curiel, AV Kajava
Journal of virology, 2008Am Soc Microbiol
There are more than 100 known adenovirus (AdV) serotypes, including 50 human
serotypes. Because AdV-induced disease is relatively species specific, vectors derived from
nonhuman serotypes may have wider clinical potential based, in part, on the lack of
ubiquitous memory immunity. Whereas a few of the human serotype capsids have been
studied at the structural level, none of the nonhuman serotypes has been analyzed. The
basis laid by the analysis of human AdV (hAdV) has allowed us to determine and compare …
Abstract
There are more than 100 known adenovirus (AdV) serotypes, including 50 human serotypes. Because AdV-induced disease is relatively species specific, vectors derived from nonhuman serotypes may have wider clinical potential based, in part, on the lack of ubiquitous memory immunity. Whereas a few of the human serotype capsids have been studied at the structural level, none of the nonhuman serotypes has been analyzed. The basis laid by the analysis of human AdV (hAdV) has allowed us to determine and compare the three-dimensional structure of the capsid of canine serotype 2 (CAV-2) to that of hAdV serotype 5 (hAdV-5). We show that CAV-2 capsid has a smoother structure than the human serotypes. Many of the external loops found in the hAdV-5 penton base and the hexon, against which the antibody response is directed, are shorter or absent in CAV-2. On the other hand, the CAV-2 fiber appears to be more complex, with two bends in the shaft. An interesting difference between the human and canine viruses is that the C-terminal part of protein IX is in a different position, making an antenna sticking out of the CAV-2 capsid. The comparison between the two viruses allows the identification of sites that should be easy to modify on the CAV-2 capsid for altering tissue tropism or other biological activities.
American Society for Microbiology