PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR
OJ Marshall - Bioinformatics, 2004 - academic.oup.com
Bioinformatics, 2004•academic.oup.com
PerlPrimer is a cross-platform graphical user interface application for the design of primers
for standard, bisulphite and real-time PCR, and sequencing. The program incorporates
highly accurate melting-temperature and primer–dimer prediction algorithms with powerful
tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs.
It aims to automate and simplify the process of primer design. Availability: Open-source and
freely available from http://perlprimer. sourceforge. net
for standard, bisulphite and real-time PCR, and sequencing. The program incorporates
highly accurate melting-temperature and primer–dimer prediction algorithms with powerful
tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs.
It aims to automate and simplify the process of primer design. Availability: Open-source and
freely available from http://perlprimer. sourceforge. net
Abstract
Summary: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer–dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.
Availability: Open-source and freely available from http://perlprimer.sourceforge.net
Oxford University Press