ST2/T1 protein functionally binds to two secreted proteins from Balb/c 3T3 and human umbilical vein endothelial cells but does not bind interleukin 1
S Kumar, MD Minnich, PR Young - Journal of Biological Chemistry, 1995 - ASBMB
The ST2/T1 receptor, a homologue of the interleukin 1 receptor (IL-1R), was expressed in
COS and Drosophila S2 cells as a human IgG-Fc fusion protein. While a type I IL-1RFc
fusion protein bound human IL-1 in vitro, the ST2Fc fusion protein did not. Furthermore, IL-1
stimulated a synthetic interleukin-8 promoter reporter gene that was cotransfected into Jurkat
cells with a full-length IL-1R type I (IL-1R1) or a chimeric receptor composed of the IL-1RI
extracellular domain and ST2 intracellular domain. In contrast, IL-1 did not stimulate the …
COS and Drosophila S2 cells as a human IgG-Fc fusion protein. While a type I IL-1RFc
fusion protein bound human IL-1 in vitro, the ST2Fc fusion protein did not. Furthermore, IL-1
stimulated a synthetic interleukin-8 promoter reporter gene that was cotransfected into Jurkat
cells with a full-length IL-1R type I (IL-1R1) or a chimeric receptor composed of the IL-1RI
extracellular domain and ST2 intracellular domain. In contrast, IL-1 did not stimulate the …
