(A) The plots show the frequency of CD11c⁺CD1a⁺CD5⁺ and CD11c⁺CD1c⁺CD5⁺ DCs obtained from cultures of cord blood CD34⁺CD117⁺CD123^– cells with GM-CSF (GM), SCF, and FLT3-L (FL) on day 7. (B) The graph shows the number of CD11c⁺CD1c⁺CD5⁺ DCs on day 7 of culturing CD34⁺ HPCs with indicated cytokines (n = 6). (C) Day 12 in vitro CD1c⁺CD5⁺, CD1c⁺CD5^–, or CD14⁺ DCs were sorted and cocultured with naive T cells. The graph shows the number of CD8⁺ T cells that diluted CFSE in response to different DC subsets from the different culture conditions. (D) Sorted in vitro DC subsets were cocultured with naive allogeneic CFSE-labeled T cells for 7 days (300 DCs: 1 × 10⁵ T cells). CFSE^lo cells were analyzed for the expression of granzyme B and perforin. One of three experiments is shown. (E) Similar to D, the plot shows the expression of IFN-γ and TNF-α by naive CD8⁺ T cells that were primed by each in vitro DC subsets. One of 3 experiments is shown. (F) Similar to C, the graph shows the number of CD4⁺ T cells that diluted CFSE in response to different to different DC subsets from the different culture conditions. (G) Similar to D, the plot shows the fraction of CD4⁺ T cells that diluted CFSE and produced IL-22 following 6 days of priming with the different DCs subsets. One of 3 experiments is shown. (H) The plot shows IL-22 and IFN-γ production by CFSE^loCD4⁺ T cells that were primed by each in vitro DC following reactivation by anti-CD3 and anti-CD28 mAbs for 18 hours (n = 6 for CD5⁺ and CD5^–; n = 3 for CD14⁺ DCs). Data represent mean ± SEM (B, C, F, and H); *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001 by paired Student’s t tests.