TY - JOUR AU - Tischner, Denise AU - Grimm, Myriam AU - Kaur, Harmandeep AU - Staudenraus, Daniel AU - Carvalho, Jorge AU - Looso, Mario AU - Günther, Stefan AU - Wanke, Florian AU - Moos, Sonja AU - Siller, Nelly AU - Breuer, Johanna AU - Schwab, Nicholas AU - Zipp, Frauke AU - Waisman, Ari AU - Kurschus, Florian C. AU - Offermanns, Stefan AU - Wettschureck, Nina T1 - Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation PY - 2017/08/03/ AB - GPCR expression was intensively studied in bulk cDNA of leukocyte populations, but limited data are available with respect to expression in individual cells. Here, we show a microfluidic-based single-cell GPCR expression analysis in primary T cells, myeloid cells, and endothelial cells under naive conditions and during experimental autoimmune encephalomyelitis, the mouse model of multiple sclerosis. We found that neuroinflammation induces characteristic changes in GPCR heterogeneity and patterning, and we identify various functionally relevant subgroups with specific GPCR profiles among spinal cord–infiltrating CD4 T cells, macrophages, microglia, or endothelial cells. Using GPCRs CXCR4, S1P1, and LPHN2 as examples, we show how this information can be used to develop new strategies for the functional modulation of Th17 cells and activated endothelial cells. Taken together, single-cell GPCR expression analysis identifies functionally relevant subpopulations with specific GPCR repertoires and provides a basis for the development of new therapeutic strategies in immune disorders. JF - JCI Insight JA - JCI Insight SN - 2379-3708 DO - 10.1172/jci.insight.95063 VL - 2 IS - 15 UR - https://doi.org/10.1172/jci.insight.95063 PB - The American Society for Clinical Investigation ER -