TY - JOUR AU - Khan, Abdul Waheed AU - Ziemann, Mark AU - Corcoran, Susan J. AU - K.N, Harikrishnan AU - Okabe, Jun AU - Rafehi, Haloom AU - Maxwell, Scott S. AU - Esler, Murray D. AU - El-Osta, Assam T1 - NET silencing by let-7i in postural tachycardia syndrome PY - 2017/03/23/ AB - While strongly implicated in postural tachycardia syndrome (POTS), considerable controversy exists regarding norepinephrine transporter (NET) loss of function. POTS is characterized by the clinical symptoms of orthostatic intolerance, lightheadedness, tachycardia, and syncope or near syncope with upright posture. Abnormal sympathetic nervous system activity is typical, of a type which suggests dysfunction of the NET, with evidence that the gene responsible is under tight epigenetic control. Using RNA of isolated chromatin combined with massive parallel sequencing (RICh-seq) we show that let-7i miRNA suppresses NET by methyl-CpG-binding protein 2 (MeCP2). Vorinostat restores epigenetic control and NET expression in leukocytes derived from POTS participants. JF - JCI Insight JA - JCI Insight SN - 2379-3708 DO - 10.1172/jci.insight.90183 VL - 2 IS - 6 UR - https://doi.org/10.1172/jci.insight.90183 PB - The American Society for Clinical Investigation ER -