TY - JOUR AU - Gheinani, Ali Hashemi AU - Kiss, Bernhard AU - Moltzahn, Felix AU - Keller, Irene AU - Bruggmann, Rémy AU - Rehrauer, Hubert AU - Fournier, Catharine Aquino AU - Burkhard, Fiona C. AU - Monastyrskaya, Katia T1 - Characterization of miRNA-regulated networks, hubs of signaling, and biomarkers in obstruction-induced bladder dysfunction PY - 2017/01/26/ AB - Bladder outlet obstruction (BOO) induces significant organ remodeling, leading to lower urinary tract symptoms accompanied by urodynamic changes in bladder function. Here, we report mRNA and miRNA transcriptome sequencing of bladder samples from human patients with different urodynamically defined states of BOO. Patients’ miRNA and mRNA expression profiles correlated with urodynamic findings. Validation of RNA sequencing results in an independent patient cohort identified combinations of 3 mRNAs (NRXN3, BMP7, UPK1A) and 3 miRNAs (miR-103a-3p, miR-10a-5p, miR-199a-3p) sufficient to discriminate between bladder functional states. All BOO patients shared cytokine and immune response pathways, TGF-β and NO signaling pathways, and hypertrophic PI3K/AKT signaling pathways. AP-1 and NFkB were dominant transcription factors, and TNF-α was the top upstream regulator. Integrated miRNA-mRNA expression analysis identified pathways and molecules targeted by differentially expressed miRNAs. Molecular changes in BOO suggest an increasing involvement of miRNAs in the control of bladder function from the overactive to underactive/acontractile states. JF - JCI Insight JA - JCI Insight SN - 2379-3708 DO - 10.1172/jci.insight.89560 VL - 2 IS - 2 UR - https://doi.org/10.1172/jci.insight.89560 PB - The American Society for Clinical Investigation ER -