(A) CFZ concentrations following 30-minute infusion (n = 45). (B) CFZ effluent from HAI pump. (C) Left: Perfusion schema. Right: Pre- and posthepatic CFZ concentrations. (D) Bright-field images and IC₅₀ curves for PDO-1 (top) and PDO-2 (bottom) at 10 nM CFZ for 1 hour or 96 hours. Note morphology (yellow arrows). Scale bars: 200 μm. (E and F) Proteasome activity and cell viability after 1 hour of 30 nM CFZ in PDO-1 (E) and PDO-2 (F). (G and H) Proteasome activity and cell viability after 48 hours of 30 nM CFZ in PDO-1 (G) and PDO-2 (H). Color indicates replicates. (I) Left: Perfusion schema. Right: Proteasome activity in tumor at 0 hours and 48 hours of perfusion with CFZ. (J) H&E and cleaved caspase-3 (CC3) staining at 0 hours and 48 hours of CFZ treatment. The box-and-whisker plot shows the median (line in box), 25th and 75th percentiles (box bounds), and minimum/maximum (whiskers). n = 4. Right: Average of CC3-positive nuclei (original magnification, ×10). Mean ± SD, n = 3. Scale bars: 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA followed by Dunnett’s post hoc test (E–H and J) or 2-tailed Student’s t test (I).