(A–C) Kv11.1 channel activation kinetics. (A) Typical current traces in Kv11.1 with holding potentials to assess time constant of activation. Tail currents were normalized to the max current at –100 mV step. (B) Current-time plot showing normalized current (I/I_max) from peak tail currents at –100 mV step after increasing durations of depolarizing step potentials. Solid lines represent single-phase exponential decay function fit to data. (C) Time constants of activation (τ) measured from individual cells in the presence of vehicle (n = 4) or evacetrapib (n = 6). **P < 0.01 by 2-tailed Student’s t test. (D–F) Kv11.1 channel deactivation kinetics. (D) Typical current traces in Kv11.1 measured at step potential to 120 mV. Gray boxed inset shows enlarged tail currents. Black arrows show the area of double exponential decay fit to measure deactivation rates of the fast (τ_f) and slow (τ_s) time components. (E and F) Rate constant measurements for the (E) fast and (F) slow component of deactivation in Kv11.1 cells held at different voltages in the presence of vehicle (n = 7) or evacetrapib (n = 8). *P < 0.05 by multiple t tests with 2-stage step up with Benjamini, Krieger, and Yekutieli. All current traces and analyses in the presence of vehicle (0.1% DMSO, black) or evacetrapib (15 μmol/L, blue) in bath solution. All data are reported as mean ± SD.