Cell-free DNA methylomics identify tissue injury patterns in pediatric ARDS
Nadir Yehya, Jacob E. Till, Nishi Srivastava, Donglan Zhang, Jason D. Christie, Erica L. Carpenter, Nilam S. Mangalmurti, Wanding Zhou
Nadir Yehya, Jacob E. Till, Nishi Srivastava, Donglan Zhang, Jason D. Christie, Erica L. Carpenter, Nilam S. Mangalmurti, Wanding Zhou
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Research Letter Inflammation Pulmonology

Cell-free DNA methylomics identify tissue injury patterns in pediatric ARDS

Abstract

Authors

Nadir Yehya, Jacob E. Till, Nishi Srivastava, Donglan Zhang, Jason D. Christie, Erica L. Carpenter, Nilam S. Mangalmurti, Wanding Zhou

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Figure 1

Comparing methylation (837 CpG probes) of isolated reference cells, ARDS, and controls.

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Comparing methylation (837 CpG probes) of isolated reference cells, ARDS...
Myeloid cells dominate most profiles, with 2 distinct subgroups: a larger group with mainly granulocytes (potentially NETosis) and a smaller group enriched for monocyte. In 1 CART19-treated patient, a B cell cfDNA signal was observed, consistent with CART-19 targeting B cells. Several patients with ARDS and controls displayed signal from liver cells, suggesting liver damage. A neuron/glial cell signal was detected in 1 sample, potentially reflecting brain injury, and 1 sample exhibited a strong signal from erythrocyte progenitors.