@article{10.1172/jci.insight.148960, author = {May Wathone Oo AND Hotaka Kawai AND Kiyofumi Takabatake AND Shuta Tomida AND Takanori Eguchi AND Kisho Ono AND Qiusheng Shan AND Toshiaki Ohara AND Saori Yoshida AND Haruka Omori AND Shintaro Sukegawa AND Keisuke Nakano AND Kuniaki Okamoto AND Akira Sasaki AND Hitoshi Nagatsuka}, journal = {JCI Insight}, publisher = {The American Society for Clinical Investigation}, title = {Resident stroma-secreted chemokine CCL2 governs myeloid-derived suppressor cells in the tumor microenvironment}, year = {2022}, month = {2}, volume = {7}, url = {https://insight.jci.org/articles/view/148960}, abstract = {Accumulating evidence has shown that cancer stroma and BM-derived cells (BMDCs) in the tumor microenvironment (TME) play vital roles in tumor progression. However, the mechanism by which oral cancer stroma recruits any particular subset of BMDCs remains largely unknown. Here, we sought to identify the subset of BMDCs that is recruited by cancer stroma. We established a sequential transplantation model in BALB/c nude mice, including (a) BM transplantation of GFP-expressing cells and (b) coxenografting of patient-derived stroma (PDS; 2 cases, designated PDS1 and PDS2) with oral cancer cells (HSC-2). As controls, xenografting was performed with HSC-2 alone or in combination with normal human dermal fibroblasts (HDF). PDS1, PDS2, and HDF all promoted BMDC migration in vitro and recruitment in vivo. Multicolor immunofluorescence revealed that the PDS coxenografts recruited Arginase-1+CD11b+GR1+GFP+ cells, which are myeloid-derived suppressor cells (MDSCs), to the TME, whereas the HDF coxenograft did not. Screening using microarrays revealed that PDS1 and PDS2 expressed CCL2 mRNA (encoding C-C motif chemokine ligand 2) at higher levels than did HDF. Indeed, PDS xenografts contained significantly higher proportions of CCL2+ stromal cells and CCR2+Arginase-1+CD11b+GR1+ MDSCs (as receiver cells) than the HDF coxenograft. Consistently, a CCL2 synthesis inhibitor and a CCR2 antagonist significantly inhibited the PDS-driven migration of BM cells in vitro. Furthermore, i.p. injection of the CCR2 antagonist to the PDS xenograft models significantly reduced the CCR2+Arginase-1+CD11b+GR1+ MDSC infiltration to the TME. In conclusion, oral cancer stroma–secreted CCL2 is a key signal for recruiting CCR2+ MDSCs from BM to the TME.}, number = {1}, doi = {10.1172/jci.insight.148960}, url = {https://doi.org/10.1172/jci.insight.148960}, }