TY - JOUR AU - Voss, Courtney AU - Esmail, Sally AU - Liu, Xuguang AU - Knauer, Michael J. AU - Ackloo, Suzanne AU - Kaneko, Tomonori AU - Lowes, Lori AU - Stogios, Peter AU - Seitova, Almagul AU - Hutchinson, Ashley AU - Yusifov, Farhad AU - Skarina, Tatiana AU - Evdokimova, Elena AU - Loppnau, Peter AU - Ghiabi, Pegah AU - Haijan, Taraneh AU - Zhong, Shanshan AU - Abdoh, Husam AU - Hedley, Benjamin D. AU - Bhayana, Vipin AU - Martin, Claudio M. AU - Slessarev, Marat AU - Chin-Yee, Benjamin AU - Fraser, Douglas D. AU - Chin-Yee, Ian AU - Li, Shawn S.C. T1 - Epitope-specific antibody responses differentiate COVID-19 outcomes and variants of concern PY - 2021/07/08/ AB - BACKGROUND The role of humoral immunity in COVID-19 is not fully understood, owing, in large part, to the complexity of antibodies produced in response to the SARS-CoV-2 infection. There is a pressing need for serology tests to assess patient-specific antibody response and predict clinical outcome.METHODS Using SARS-CoV-2 proteome and peptide microarrays, we screened 146 COVID-19 patients’ plasma samples to identify antigens and epitopes. This enabled us to develop a master epitope array and an epitope-specific agglutination assay to gauge antibody responses systematically and with high resolution.RESULTS We identified linear epitopes from the spike (S) and nucleocapsid (N) proteins and showed that the epitopes enabled higher resolution antibody profiling than the S or N protein antigen. Specifically, we found that antibody responses to the S-811–825, S-881–895, and N-156–170 epitopes negatively or positively correlated with clinical severity or patient survival. Moreover, we found that the P681H and S235F mutations associated with the coronavirus variant of concern B.1.1.7 altered the specificity of the corresponding epitopes.CONCLUSION Epitope-resolved antibody testing not only affords a high-resolution alternative to conventional immunoassays to delineate the complex humoral immunity to SARS-CoV-2 and differentiate between neutralizing and non-neutralizing antibodies, but it also may potentially be used to predict clinical outcome. The epitope peptides can be readily modified to detect antibodies against variants of concern in both the peptide array and latex agglutination formats.FUNDING Ontario Research Fund (ORF) COVID-19 Rapid Research Fund, Toronto COVID-19 Action Fund, Western University, Lawson Health Research Institute, London Health Sciences Foundation, and Academic Medical Organization of Southwestern Ontario (AMOSO) Innovation Fund. JF - JCI Insight JA - JCI Insight SN - 2379-3708 DO - 10.1172/jci.insight.148855 VL - 6 IS - 13 UR - https://doi.org/10.1172/jci.insight.148855 PB - The American Society for Clinical Investigation ER -