Go to The Journal of Clinical Investigation
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Immunology
    • Metabolism
    • Nephrology
    • Oncology
    • Pulmonology
    • All ...
  • Videos
  • Collections
    • Resource and Technical Advances
    • Clinical Medicine
    • Reviews
    • Editorials
    • Perspectives
    • Top read articles
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • In-Press Preview
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
ENT2 facilitates brain endothelial cell penetration and blood-brain barrier transport by a tumor-targeting anti-DNA autoantibody
Zahra Rattray, … , Jiangbing Zhou, James E. Hansen
Zahra Rattray, … , Jiangbing Zhou, James E. Hansen
Published June 15, 2021
Citation Information: JCI Insight. 2021;6(14):e145875. https://doi.org/10.1172/jci.insight.145875.
View: Text | PDF
Research Article Oncology

ENT2 facilitates brain endothelial cell penetration and blood-brain barrier transport by a tumor-targeting anti-DNA autoantibody

  • Text
  • PDF
Abstract

The blood-brain barrier (BBB) prevents antibodies from penetrating the CNS and limits conventional antibody-based approaches to brain tumors. We now show that ENT2, a transporter that regulates nucleoside flux at the BBB, may offer an unexpected path to circumventing this barrier to allow targeting of brain tumors with an anti-DNA autoantibody. Deoxymab-1 (DX1) is a DNA-damaging autoantibody that localizes to tumors and is synthetically lethal to cancer cells with defects in the DNA damage response. We found that DX1 penetrated brain endothelial cells and crossed the BBB, and mechanistic studies identify ENT2 as the key transporter. In efficacy studies, DX1 crosses the BBB to suppress orthotopic glioblastoma and breast cancer brain metastases. ENT2-linked transport of autoantibodies across the BBB has potential to be exploited in brain tumor immunotherapy, and its discovery raises hypotheses on actionable mechanisms of CNS penetration by neurotoxic autoantibodies in CNS lupus.

Authors

Zahra Rattray, Gang Deng, Shenqi Zhang, Anupama Shirali, Christopher K. May, Xiaoyong Chen, Benedette J. Cuffari, Jun Liu, Pan Zou, Nicholas J.W. Rattray, Caroline H. Johnson, Valentina Dubljevic, James A. Campbell, Anita Huttner, Joachim M. Baehring, Jiangbing Zhou, James E. Hansen

×

Figure 4

DX1 suppresses tumors in 2 orthotopic PDX models of GBM.

Options: View larger image (or click on image) Download as PowerPoint
DX1 suppresses tumors in 2 orthotopic PDX models of GBM.
(A–C) DX1 suppr...
(A–C) DX1 suppresses GBM model 1 tumors. Tumor growth after inoculation of luciferase-expressing GBM model 1 GSCs into the brains of immunodeficient mice was followed by radiance efficiency in the brain by weekly IVIS. Two weeks after inoculation, mice began treatment with i.v. control buffer (group 1) or DX1 (20 mg/kg, group 2). (A) Radiance efficiencies are shown and demonstrate significant suppression of tumors by DX1 (note the y axis scale increases over the weeks). **P < 0.04, Student’s t test, n = 7 per group. *P < 0.02, Student’s t test, n = 4 and 7 per group. (B) Representative IVIS images and H&E stains are shown. Scale bar: 1.25 mm. (C) Treatment with DX1 increased median survival (measured from initiation of treatment) to 24 days, compared with 17 days in control mice (P < 0.01, log-rank test, n = 7). (D–G) DX1 suppresses GBM model 2 tumors. Two weeks after inoculation of GBM model 2 GSCs, mice began treatment with i.v. control buffer (n = 10) or 20 mg/kg DX1 (n = 10) 3 times per week. At 9 weeks after inoculation, brains from 3 mice per group were analyzed by H&E and Ki67 stains to facilitate measurement of mean tumor areas. (D and E) Representative H&E sections and mean tumor areas (*P = 0.04, Student’s t test, n = 3). Scale bar: 1.25 mm. (F) Sections of tumors from mice treated with control or DX1 stained to detect DX1 with protein L are shown and demonstrate that DX1 crossed the BBB to penetrate tumors and was associated with increased TUNEL staining. Scale bar: 50 μm. Results were confirmed by separately staining tumor sections with an anti-DX1 antibody (Supplemental Figure 4). (G) DX1 prolonged median survival in GBM model 2 to 73 days, compared with 58 days in mice treated with control (P = 0.02, log-rank test, n = 7).

Copyright © 2023 American Society for Clinical Investigation
ISSN 2379-3708

Sign up for email alerts