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Lymphocyte mass cytometry identifies a CD3–CD4+ cell subset with a potential role in psoriasis
Ruru Guo, … , Xiaoxiang Chen, Liangjing Lu
Ruru Guo, … , Xiaoxiang Chen, Liangjing Lu
Published February 12, 2019
Citation Information: JCI Insight. 2019;4(6):e125306. https://doi.org/10.1172/jci.insight.125306.
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Research Article Inflammation

Lymphocyte mass cytometry identifies a CD3–CD4+ cell subset with a potential role in psoriasis

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Abstract

Psoriasis (PS) is a systemic, immune-mediated inflammatory disorder. However, the whole lymphocyte compartment and the potential pathologies of PS have not been fully characterized. In the present study, we examined whole lymphocyte subsets and signal transduction proteins using high-dimensional single-cell mass cytometry and a bioinformatics pipeline for an in-depth characterization of the immune cell subsets and protein profiles involved in pathways in the peripheral blood of patients with PS. We identified 15 major immune cell populations in T cell lineages and characterized various CD3+CD4+ Th and CD3+CD8+ T cytotoxic cell populations simultaneously across 24 leukocyte markers and 7 proteins related to the signal transduction pathways. High-dimensional analysis identified 3 new subsets that are abundant in PS peripheral blood, resembling CD3–CD4+ lymphoid tissue inducer cells, Tc17 cells, and CD8+CXCR3+ Tregs. We confirmed the CD3–CD4+ cells, and their features and functions, in an independent PS cohort. The use of single-cell mass cytometry allows systemic-level characterization of lymphocyte subpopulations and dysregulated signaling pathways in the blood of patients with PS, identifying abnormalities of different immune cell subsets. We validated that the CD3–CD4+ cells had elevated OX40 and decreased FRA2 expression, which were positively associated with the PS area and severity index.

Authors

Ruru Guo, Ting Zhang, Xinyu Meng, Zhen Lin, Jinran Lin, Yu Gong, Xuesong Liu, Yuetian Yu, Guilin Zhao, Xianting Ding, Xiaoxiang Chen, Liangjing Lu

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Figure 8

Function assay of CD3–CD4+ cells in PS.

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Function assay of CD3–CD4+ cells in PS.
(A) Serum concentrations of OX40...
(A) Serum concentrations of OX40L in HC (n = 15) and PS patients (n = 31). The data are illustrated as the mean ± SD and analyzed by 2-tailed Student’s t test. (B) The changes of CD3–CD4+cells, CD3–CD4+OX40+, and CD3–CD4+Fra2+cells in PBMCs with CVN treatment in PS (n = 18) were analyzed by 2-tailed Student’s t test. (C) After 36 hours of treatment with CVN, the trend of the CD3–CD4+, CD3–CD4+OX40+, and CD3–CD4+Fra2+ cell percentage in PBMCs was shown (n = 18). (D) There was positive correlation between CD3–CD4+cells and PASI before CVN treatment (n = 18) by Pearson correlation analysis. Correlation analysis was performed using the Pearson’s correlation. **P < 0.005; ***P < 0.0005; ****P < 0.0001.

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