(A) Magnetically sorted IPF CCR10^hi and CCR10^lo cells were intravenously administered into NSG mice (0.25 million cells/mouse) and after 35 days, the mice were sacrificed, their lungs were collected for histological, biochemical, and flow cytometric analyses. (B) Shown is an image of IPF CCR10^hi-humanized NSG mice, 31 days after cellular administration. (C) Shown are representative images of lung tissues, from naive (left), IPF CCR10^hi-humanized (middle), and IPF CCR10^lo-humanized (right) NSG groups taken at ×100 magnification. (D) Depicted is the hydroxyproline concentration in the lungs of naive, nonhumanized, or humanized NSG mice that received IPF CCR10^hi or CCR10^lo cells. n = 5–10 mice/group. Data shown are the mean ± SEM. ns, not significant; *P ≤ 0.05 via 1-way ANOVA corrected for multiple comparison via Dunnett’s test. (E) CCR10^hi or CCR10^lo humanized NSG mouse lung suspensions were analyzed by flow cytometry for human CD45, EpCAM, and/or CCR10 expression. Depicted are representative dot plots showing EpCAM⁺ cells (left) and the percentage of EpCAM⁺ cells expressing cell surface CCR10 (right) in IPF CCR10^hi (top) and CCR10^lo (bottom) humanized NSG lungs. (F) Shown is the average number of EpCAM⁺CCR10⁺ cells in IPF CCR10^hi and CCR10^lo humanized NSG mice. n = 5/group. Data shown are the mean ± SEM. *P ≤ 0.05 via 2-tailed Mann-Whitney nonparametric test. (G) IPF lung explant cells were stained with anti-CD45, anti-EpCAM, and anti-CCR10 antibodies. Shown is the mean GMFI for CCR10 staining on CD45⁺ and CD45^–EpCAM⁺ cells. n = 12/group. Data shown are the mean ± SEM. ****P ≤ 0.0001 via 2-tailed Mann-Whitney nonparametric test. IPF, idiopathic pulmonary fibrosis; NSG, NOD Cg-Prkdc^SCID IL2rg^Tm1wilSzi.