The impact of Nucleofection® on the activation state of primary human CD4 T cells
M Zhang, Z Ma, N Selliah, G Weiss, A Genin… - Journal of …, 2014 - Elsevier
Gene transfer into primary human CD4 T lymphocytes is a critical tool in studying the
mechanism of T cell-dependent immune responses and human immunodeficiency virus-1
(HIV-1) infection. Nucleofection® is an electroporation technique that allows efficient gene
transfer into primary human CD4 T cells that are notoriously resistant to traditional
electroporation. Despite its popularity in immunological research, careful characterization of
its impact on the physiology of CD4 T cells has not been documented. Herein, using freshly …
mechanism of T cell-dependent immune responses and human immunodeficiency virus-1
(HIV-1) infection. Nucleofection® is an electroporation technique that allows efficient gene
transfer into primary human CD4 T cells that are notoriously resistant to traditional
electroporation. Despite its popularity in immunological research, careful characterization of
its impact on the physiology of CD4 T cells has not been documented. Herein, using freshly …