DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification …

WJ Swiggard, A Mirza, MC Nussenzweig… - Cellular …, 1995 - Elsevier
The monoclonal antibody NLDC-145 detects an antigen expressed at high levels by mouse
dendritic cells (DCs) and thymic epithelial cells. Here we report on the purification and
biochemical characterization of this antigen. The antigen detected by the NLDC-145 mAb is
an integral membrane glycoprotein, with an apparent molecular mass of 205 kDa by
immunoprecipitation and Western blotting. The isoelectric point is pH 7.5, and carbohydrates
comprise about 7 kDa of the total mass. Lectin blotting and FACE analysis revealed …