A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and
fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining,
Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-
linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells,
pathogens, proteins such as cytokines/chemokines, and the expression levels of
inflammation-related and other genes in the cells. This will help to understand the …