An approach to study melanoma invasion and crosstalk with lymphatic endothelial cell spheroids in 3D using immunofluorescence
S Alve, S Gramolelli, PM Ojala - Melanoma: Methods and Protocols, 2021 - Springer
S Alve, S Gramolelli, PM Ojala
Melanoma: Methods and Protocols, 2021•SpringerAbstract Three-dimensional (3D) cell culture has allowed a deeper understanding of
complex pathological and physiological processes, overcoming some of the limitations of 2D
cell culture on plastic and avoiding the costs and ethical issues related to experiments
involving animals. Here we describe a protocol to embed single melanoma cells alone or
together with primary human lymphatic endothelial cells in a 3D cross-linked matrix, to
investigate the invasion and molecular crosstalk between these two cell types, respectively …
complex pathological and physiological processes, overcoming some of the limitations of 2D
cell culture on plastic and avoiding the costs and ethical issues related to experiments
involving animals. Here we describe a protocol to embed single melanoma cells alone or
together with primary human lymphatic endothelial cells in a 3D cross-linked matrix, to
investigate the invasion and molecular crosstalk between these two cell types, respectively …
Abstract
Three-dimensional (3D) cell culture has allowed a deeper understanding of complex pathological and physiological processes, overcoming some of the limitations of 2D cell culture on plastic and avoiding the costs and ethical issues related to experiments involving animals. Here we describe a protocol to embed single melanoma cells alone or together with primary human lymphatic endothelial cells in a 3D cross-linked matrix, to investigate the invasion and molecular crosstalk between these two cell types, respectively. After fixation and staining with antibodies and fluorescent conjugates, phenotypic changes in both cell types can be specifically analyzed by confocal microscopy.
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