Establishment and evaluation of 2 monoclonal antibodies against oxidized apolipoprotein AI (apoA-I) and its application to determine blood oxidized apoA-I levels

M Ueda, Y Hayase, S Mashiba - Clinica chimica acta, 2007 - Elsevier
M Ueda, Y Hayase, S Mashiba
Clinica chimica acta, 2007•Elsevier
BACKGROUND: Apolipoprotein AI (apoA-I) is the major lipoprotein component of high-
density lipoprotein (HDL), and plays an important role in reverse cholesterol transport. Its
function is known to be influenced by oxidation. METHODS: Using H2O2-or chloramine T-
oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established
an ELISA system for the measurement of oxidized apoA-I. RESULTS: The 2 monoclonal
antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum …
BACKGROUND
Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation.
METHODS
Using H2O2- or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I.
RESULTS
The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I.
CONCLUSION
The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.
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