[HTML][HTML] Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
STAR protocols, 2022•Elsevier
Genetic variants that affect neurological function will often produce changes visible at the
level of gross morphology, either of the whole brain or of specific neuronal types. Here we
describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we
outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic
arborization (Sholl analysis). For complete details on the use and execution of this protocol,
please refer to Gennarino et al.(2018).
level of gross morphology, either of the whole brain or of specific neuronal types. Here we
describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we
outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic
arborization (Sholl analysis). For complete details on the use and execution of this protocol,
please refer to Gennarino et al.(2018).
Summary
Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic arborization (Sholl analysis).
For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018).
Elsevier
