[HTML][HTML] The landscape of T and B lymphocytes interaction and synergistic effects of Th1 and Th2 type response in the involved tissue of IgG4-RD revealed by single …
S Cai, Y Chen, Z Hu, T Zhou, Y Huang, S Lin… - Journal of …, 2022 - Elsevier
S Cai, Y Chen, Z Hu, T Zhou, Y Huang, S Lin, R Gao, J Zhong, L Dong
Journal of Autoimmunity, 2022•ElsevierObjectives To investigate the landscape of TB cell interaction, immune receptor profiles and
effects of different types of immune responses in the involved tissues of IgG4-RD. Methods
Single cell RNA sequencing, bulk sample RNA sequencing, immune receptor repertoire
analysis (both BCR and TCR), multi-color flow cytometry, and in-vitro assays with model
cells (eg EBV-immortalized B cells from IgG4-RD patient) and histologic methods were
applied to investigate the immunopathological features of IgG4-RD from multiple aspects …
effects of different types of immune responses in the involved tissues of IgG4-RD. Methods
Single cell RNA sequencing, bulk sample RNA sequencing, immune receptor repertoire
analysis (both BCR and TCR), multi-color flow cytometry, and in-vitro assays with model
cells (eg EBV-immortalized B cells from IgG4-RD patient) and histologic methods were
applied to investigate the immunopathological features of IgG4-RD from multiple aspects …
Objectives
To investigate the landscape of T-B cell interaction, immune receptor profiles and effects of different types of immune responses in the involved tissues of IgG4-RD.
Methods
Single cell RNA sequencing, bulk sample RNA sequencing, immune receptor repertoire analysis (both BCR and TCR), multi-color flow cytometry, and in-vitro assays with model cells (e.g. EBV-immortalized B cells from IgG4-RD patient) and histologic methods were applied to investigate the immunopathological features of IgG4-RD from multiple aspects.
Results
Ectopic germinal center formation was observed in IgG4-RD patients at advanced disease stage, and a large part of B cells in involved tissue were germinal center B cell-like. Germinal center reaction in IgG4-RD led to the irregularities of both TCR and BCR clones in the involved tissues, and limited clonal overlaps among different samples. Enhanced Th1- and Th2-type responses were observed in involved tissues of IgG4-RD and patients with both increased Th1- and Th2-type response related cell subsets possessed more severe inflammatory indices. Analyses to the origin of IGHG4 transcripts in IgG4-RD indicated that IgG4 could be switched from IgM directly, or from other IgG subclasses. In vitro assays with EBV-immortalized B cells, fibroblasts and epithelial cells revealed the effects of Th1-type and Th2-type responses on germinal center reaction, ectopic expression of MHC-II molecules, and formation of tertiary lymphoid structures.
Conclusions
Synergistic effects of Th1- and Th2-type responses were involved in the pathogenesis of IgG4-RD via their influences on both acute inflammatory processes and the chronicity and complexity of IgG4-RD.
Elsevier