Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins
AP Heinen, F Wanke, S Moos, S Attig, H Luche… - Cytometry Part …, 2014 - Wiley Online Library
Cytometry Part A, 2014•Wiley Online Library
Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins
is hindered by loss of fluorescence using current available methods. In this study, it is shown
that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that
fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a
simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining
while retaining FPs inside fixed cells.© 2014 International Society for Advancement of …
is hindered by loss of fluorescence using current available methods. In this study, it is shown
that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that
fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a
simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining
while retaining FPs inside fixed cells.© 2014 International Society for Advancement of …
Abstract
Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells. © 2014 International Society for Advancement of Cytometry
Wiley Online Library