Role of immunoglobulin M and A antibodies in the neutralization of severe acute respiratory syndrome coronavirus 2
J Klingler, S Weiss, V Itri, X Liu… - The Journal of …, 2021 - academic.oup.com
The Journal of infectious diseases, 2021•academic.oup.com
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected
millions of people globally. Virus infection requires the receptor-binding domain (RBD) of the
spike protein. Although studies have demonstrated anti-spike and-RBD antibodies to be
protective in animal models, and convalescent plasma as a promising therapeutic option,
little is known about immunoglobulin isotypes capable of blocking infection. Methods We
studied spike-and RBD-specific immunoglobulin isotypes in convalescent and acute …
millions of people globally. Virus infection requires the receptor-binding domain (RBD) of the
spike protein. Although studies have demonstrated anti-spike and-RBD antibodies to be
protective in animal models, and convalescent plasma as a promising therapeutic option,
little is known about immunoglobulin isotypes capable of blocking infection. Methods We
studied spike-and RBD-specific immunoglobulin isotypes in convalescent and acute …
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people globally. Virus infection requires the receptor-binding domain (RBD) of the spike protein. Although studies have demonstrated anti-spike and -RBD antibodies to be protective in animal models, and convalescent plasma as a promising therapeutic option, little is known about immunoglobulin isotypes capable of blocking infection.
Methods
We studied spike- and RBD-specific immunoglobulin isotypes in convalescent and acute plasma/serum samples using a multiplex bead assay. We also determined virus neutralization activities in plasma and serum samples, and purified immunoglobulin fractions using a vesicular stomatitis pseudovirus assay.
Results
Spike- and RBD-specific immunoglobulin (Ig) M, IgG1, and IgA1 were produced by all or nearly all subjects at variable levels and detected early after infection. All samples displayed neutralizing activity. Regression analyses revealed that IgM and IgG1 contributed most to neutralization, consistent with IgM and IgG fractions’ neutralization potency. IgA also exhibited neutralizing activity, but with lower potency.
Conclusion
IgG, IgM, and IgA are critical components of convalescent plasma used for treatment of coronavirus disease 2019 (COVID-19).
Oxford University Press