Galectin-3, a novel binding partner of β-catenin
T Shimura, Y Takenaka, S Tsutsumi, V Hogan… - Cancer research, 2004 - AACR
T Shimura, Y Takenaka, S Tsutsumi, V Hogan, A Kikuchi, A Raz
Cancer research, 2004•AACRAbstract Galectin-3 (gal-3), a pleiotrophic protein, is an important regulator of tumor
metastasis, which like β-catenin shuttles between the nucleus and the cytosol in a
phosphorylation-dependent manner. We report herein that β-catenin stimulation of cyclin D1
and c-myc expression is gal-3 dependent. Gal-3 binds to β-catenin/Tcf complex, colocalizes
with β-catenin in the nucleus, and induces the transcriptional activity of Tcf-4 as determined
by the TOP/FOPFLASH reporter system. We have identified the β-catenin–gal-3–binding …
metastasis, which like β-catenin shuttles between the nucleus and the cytosol in a
phosphorylation-dependent manner. We report herein that β-catenin stimulation of cyclin D1
and c-myc expression is gal-3 dependent. Gal-3 binds to β-catenin/Tcf complex, colocalizes
with β-catenin in the nucleus, and induces the transcriptional activity of Tcf-4 as determined
by the TOP/FOPFLASH reporter system. We have identified the β-catenin–gal-3–binding …
Abstract
Galectin-3 (gal-3), a pleiotrophic protein, is an important regulator of tumor metastasis, which like β-catenin shuttles between the nucleus and the cytosol in a phosphorylation-dependent manner. We report herein that β-catenin stimulation of cyclin D1 and c-myc expression is gal-3 dependent. Gal-3 binds to β-catenin/Tcf complex, colocalizes with β-catenin in the nucleus, and induces the transcriptional activity of Tcf-4 as determined by the TOP/FOPFLASH reporter system. We have identified the β-catenin–gal-3–binding sequences, which are in the NH2 and COOH termini of the proteins encompassing amino acid residues 1 to 131 and 143 to 250, respectively. These data indicate that gal-3 is a novel binding partner for β-catenin involved in the regulation of Wnt/β-catenin signaling pathway.
AACR