[HTML][HTML] Heme deficiency selectively interrupts assembly of mitochondrial complex IV in human fibroblasts: relevance to aging
Heme deficiency was studied in young and old normal human fibroblasts (IMR90).
Regardless of age, heme deficiency increased the steady-state level of oxidants and lipid
peroxidation and sensitized the cells to fluctuations in intracellular Ca 2+. Heme deficiency
selectively decreased the activity and protein content of mitochondrial complex IV
(cytochromec oxidase) by 95%, indicating a decrease in successful assembly. Complexes I–
III and catalase remained intact under conditions of heme deficiency, whereas …
Regardless of age, heme deficiency increased the steady-state level of oxidants and lipid
peroxidation and sensitized the cells to fluctuations in intracellular Ca 2+. Heme deficiency
selectively decreased the activity and protein content of mitochondrial complex IV
(cytochromec oxidase) by 95%, indicating a decrease in successful assembly. Complexes I–
III and catalase remained intact under conditions of heme deficiency, whereas …