Development of a novel flow cytometric cell-mediated cytotoxicity assay using the fluorophores PKH-26 and TO-PRO-3 iodide

AE Lee-MacAry, EL Ross, D Davies, R Laylor… - Journal of …, 2001 - Elsevier
A flow cytometric (FCM) assay has been developed for the determination of cell-mediated
cytotoxicity (CMC). In the assay, the target tumour cell population was labelled with a
membrane dye, PKH-26, prior to incubation with splenocyte effector cells. Cell death within
the target population was assessed by the addition of the viability probe TO-PRO-3 iodide
(TP3) and analysed by flow cytometry. The extent of cytotoxicity was determined by the
relative number of live target cells labelled with PKH-26 only and dead, permeabilised cells …