[HTML][HTML] Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic
DE Carvajal-Hausdorf, KA Schalper… - Laboratory …, 2015 - Elsevier
Detection of biomolecules in tissues provides contextual information and the possibility to
assess the interaction of different cell types and markers. Routine qualitative assessment of
immune-and oligonucleotide-based methods in research and the clinic has been associated
with assay variability because of lack of stringent validation and subjective interpretation of
results. As a result, the vast majority of in situ assays in clinical usage are nonquantitative
and, although useful, often of questionable scientific validity. Here, we revisit the reporters …
assess the interaction of different cell types and markers. Routine qualitative assessment of
immune-and oligonucleotide-based methods in research and the clinic has been associated
with assay variability because of lack of stringent validation and subjective interpretation of
results. As a result, the vast majority of in situ assays in clinical usage are nonquantitative
and, although useful, often of questionable scientific validity. Here, we revisit the reporters …