[HTML][HTML] Accurate quantification of site-specific acetylation stoichiometry reveals the impact of sirtuin deacetylase CobB on the E. coli acetylome
Lysine acetylation is a protein posttranslational modification (PTM) that occurs on thousands
of lysine residues in diverse organisms from bacteria to humans. Accurate measurement of
acetylation stoichiometry on a proteome-wide scale remains challenging. Most methods
employ a comparison of chemically acetylated peptides to native acetylated peptides,
however, the potentially large differences in abundance between these peptides presents a
challenge for accurate quantification. Stable isotope labeling by amino acids in cell culture …
of lysine residues in diverse organisms from bacteria to humans. Accurate measurement of
acetylation stoichiometry on a proteome-wide scale remains challenging. Most methods
employ a comparison of chemically acetylated peptides to native acetylated peptides,
however, the potentially large differences in abundance between these peptides presents a
challenge for accurate quantification. Stable isotope labeling by amino acids in cell culture …