Insulin-like growth factor-1 gene delivery may enhance the proliferation of human corpus cavernosal smooth muscle cells

M Kim, EC Hwang, IK Park, K Park - Urology, 2010 - Elsevier
OBJECTIVES: To investigate the effects of IGF-1 gene transfer in human corpus cavernosal
smooth muscle cells (HCCSMCs) ex vivo. Insulin-like growth factor-1 (IGF-1) promotes the
proliferation of penile cavernous smooth muscle cells in the rats. METHODS: A plasmid
expressing human IGF-1 was constructed by subcloning hIGF-1 into pcDNA3. 1 vector.
HCCSMCs were harvested from 3 impotent patients and cultured in vitro. The cultured
smooth muscle cells were identified by immunofluorescent staining. RNA was extracted from …