C to U RNA editing mediated by APOBEC 1 requires RNA‐binding protein RBM 47
EMBO reports, 2014•embopress.org
Abstract Cytidine (C) to Uridine (U) RNA editing is a post‐transcriptional modification that is
accomplished by the deaminase APOBEC 1 and its partnership with the RNA‐binding
protein A 1 CF. We identify and characterise here a novel RNA‐binding protein, RBM 47,
that interacts with APOBEC 1 and A1 CF and is expressed in tissues where C to U RNA
editing occurs. RBM 47 can substitute for A1 CF and is necessary and sufficient for APOBEC
1‐mediated editing in vitro. Editing is further impaired in Rbm47‐deficient mutant mice …
accomplished by the deaminase APOBEC 1 and its partnership with the RNA‐binding
protein A 1 CF. We identify and characterise here a novel RNA‐binding protein, RBM 47,
that interacts with APOBEC 1 and A1 CF and is expressed in tissues where C to U RNA
editing occurs. RBM 47 can substitute for A1 CF and is necessary and sufficient for APOBEC
1‐mediated editing in vitro. Editing is further impaired in Rbm47‐deficient mutant mice …
Abstract
Cytidine (C) to Uridine (U) RNA editing is a post‐transcriptional modification that is accomplished by the deaminase APOBEC1 and its partnership with the RNA‐binding protein A1CF. We identify and characterise here a novel RNA‐binding protein, RBM47, that interacts with APOBEC1 and A1CF and is expressed in tissues where C to U RNA editing occurs. RBM47 can substitute for A1CF and is necessary and sufficient for APOBEC1‐mediated editing in vitro. Editing is further impaired in Rbm47‐deficient mutant mice. These findings suggest that RBM47 and APOBEC1 constitute the basic machinery for C to U RNA editing.
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