Characterization of Colorectal Cancer Development in Apc min/+ Mice
ILK Nalbantoglu, V Blanc, NO Davidson - Gastrointestinal Physiology and …, 2016 - Springer
ILK Nalbantoglu, V Blanc, NO Davidson
Gastrointestinal Physiology and Diseases: Methods and Protocols, 2016•SpringerThe Apc min/+ mouse provides an excellent experimental model for studying genetic,
environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we
will describe techniques for studying colon cancer development in Apc min/+ mice on
C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including
Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of
experimental colorectal cancer. This chapter also includes protocols describing extraction …
environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we
will describe techniques for studying colon cancer development in Apc min/+ mice on
C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including
Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of
experimental colorectal cancer. This chapter also includes protocols describing extraction …
Abstract
The Apc min/+ mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apc min/+ mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.
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