The formation of cholesterol and sphingolipids into specialized liquid-ordered membrane
microdomains (rafts) has been proposed to function in the intracellular sorting and transport
of proteins and lipids. Defined by biochemical criteria, rafts resist solubilization in nonionic
detergents, enabling them to be isolated as detergent-resistant membranes (DRM). In this
study, we characterized the lipid composition of DRM from a cell model of the sphingolipid
storage disorder, Gaucher disease, in which the catabolism of the sphingolipid …