Separation and quantitation of bile acids using an isocratic solvent system for high performance liquid chromatography coupled to an evaporative light scattering …
EC Torchia, ED Labonté, LB Agellon - Analytical Biochemistry, 2001 - Elsevier
EC Torchia, ED Labonté, LB Agellon
Analytical Biochemistry, 2001•ElsevierWe developed a quantitative method for the analysis of bile acids using a high performance
liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent
system was used to resolve in a single run conjugated and unconjugated bile acid species
relevant in human and rodent physiology. The detection of various bile acids was linear over
a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and
cost-effective method for the routine analysis of a wide variety of bile acids.
liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent
system was used to resolve in a single run conjugated and unconjugated bile acid species
relevant in human and rodent physiology. The detection of various bile acids was linear over
a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and
cost-effective method for the routine analysis of a wide variety of bile acids.
We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.
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