Replication of rhesus cytomegalovirus in life-expanded rhesus fibroblasts expressing human telomerase

WLW Chang, V Kirchoff, GS Pari, PA Barry - Journal of virological methods, 2002 - Elsevier
The kinetics of rhesus cytomegalovirus (RhCMV) infection were compared in primary and
telomerase-immortalized (Telo-) rhesus fibroblasts (RF). Equivalent viral titers were
achieved with both cell types. However, the production of infectious virions was slightly
faster and plaque size was larger in Telo-RF, compared with primary cells. Comparable
RhCMV growth curves and viral susceptibility were observed using Telo-RF passaged for
different time periods in culture, whereas the ability of primary cells to support robust RhCMV …