[HTML][HTML] Establishment of a NanoBiT-based cytosolic Ca2+ sensor by optimizing calmodulin-binding motif and protein expression levels
LP Nguyen, HT Nguyen, HJ Yong, A Reyes-Alcaraz… - Molecules and cells, 2020 - Elsevier
Cytosolic Ca 2+ levels ([Ca 2+] c) change dynamically in response to inducers, repressors,
and physiological conditions, and aberrant [Ca 2+] c concentration regulation is associated
with cancer, heart failure, and diabetes. Therefore,[Ca 2+] c is considered as a good
indicator of physiological and pathological cellular responses, and is a crucial biomarker for
drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to
measure [Ca 2+] c in single cells and animal models. GECI have some advantages over …
and physiological conditions, and aberrant [Ca 2+] c concentration regulation is associated
with cancer, heart failure, and diabetes. Therefore,[Ca 2+] c is considered as a good
indicator of physiological and pathological cellular responses, and is a crucial biomarker for
drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to
measure [Ca 2+] c in single cells and animal models. GECI have some advantages over …
