[HTML][HTML] Spatiotemporal regulation of early lipolytic signaling in adipocytes
S Martin, S Okano, C Kistler… - Journal of biological …, 2009 - ASBMB
Hormone-sensitive lipase (HSL) is a key enzyme regulating the acute activation of lipolysis.
HSL functionality is controlled by multiple phosphorylation events, which regulate its
association with the surface of lipid droplets (LDs). We determined the progression and
stability of HSL phosphorylation on individual serine residues both spatially and temporally
in adipocytes using phospho-specific antibodies. Within seconds of β-adrenergic receptor
activation, HSL was phosphorylated on Ser-660, the phosphorylated form appearing in the …
HSL functionality is controlled by multiple phosphorylation events, which regulate its
association with the surface of lipid droplets (LDs). We determined the progression and
stability of HSL phosphorylation on individual serine residues both spatially and temporally
in adipocytes using phospho-specific antibodies. Within seconds of β-adrenergic receptor
activation, HSL was phosphorylated on Ser-660, the phosphorylated form appearing in the …
