Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to …
VL Battula, PM Bareiss, S Treml, S Conrad, I Albert… - Differentiation, 2007 - Elsevier
Conventionally, mesenchymal stem cells (MSC) are generated by plating cells from bone
marrow (BM) or other sources into culture flasks and selecting plastic-adherent cells with
fibroblastoid morphology. These cells express CD9, CD10, CD13, CD73, CD105, CD166,
and other markers but show only a weak or no expression of the embryonic markers stage-
specific embryonic antigen-4 (SSEA-4), Oct-4 and nanog-3. Using a novel protocol we
prepared MSC from BM and non-amniotic placenta (PL) by culture of Ficoll-selected cells in …
marrow (BM) or other sources into culture flasks and selecting plastic-adherent cells with
fibroblastoid morphology. These cells express CD9, CD10, CD13, CD73, CD105, CD166,
and other markers but show only a weak or no expression of the embryonic markers stage-
specific embryonic antigen-4 (SSEA-4), Oct-4 and nanog-3. Using a novel protocol we
prepared MSC from BM and non-amniotic placenta (PL) by culture of Ficoll-selected cells in …