Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes
PJ Siska, B Kim, X Ji, MD Hoeksema… - Journal of …, 2016 - Elsevier
T and B lymphocytes undergo metabolic re-programming upon activation that is essential to
allow bioenergetics, cell survival, and intermediates for cell proliferation and function. To
support changes in the activity of signaling pathways and to provide sufficient and necessary
intracellular metabolites, uptake of extracellular nutrients increases sharply with metabolic re-
programming. One result of increased metabolic activity can be reactive oxygen species
(ROS), which can be toxic when accumulated in excess. Uptake of cystine allows …
allow bioenergetics, cell survival, and intermediates for cell proliferation and function. To
support changes in the activity of signaling pathways and to provide sufficient and necessary
intracellular metabolites, uptake of extracellular nutrients increases sharply with metabolic re-
programming. One result of increased metabolic activity can be reactive oxygen species
(ROS), which can be toxic when accumulated in excess. Uptake of cystine allows …