Energy state of chondrocytes assessed by 31P-NMR studies of preosseous cartilage
P Pollesello, B de Bernard, M Grandolfo… - Biochemical and …, 1991 - Elsevier
P Pollesello, B de Bernard, M Grandolfo, S Paoletti, F Vittur, BJ Kvam
Biochemical and biophysical research communications, 1991•ElsevierThe energy state of resting and hypertrophic chondrocytes from growth plate was studied by
31 P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine
was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline
in the nucleoside triphosphate level after adding blockers of the glycolysis or of the
mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use
both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic …
31 P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine
was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline
in the nucleoside triphosphate level after adding blockers of the glycolysis or of the
mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use
both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic …
Summary
The energy state of resting and hypertrophic chondrocytes from growth plate was studied by 31P-NMR spectroscopy of superfused cartilage slices. The presence of phosphocreatine was demonstrated in both cell types, using a repetition time of 3 s. By comparing the decline in the nucleoside triphosphate level after adding blockers of the glycolysis or of the mitochondrial respiration, it was deduced that resting and hypertrophic chondrocytes use both metabolic pathways for energy production, but the glycolysis dominates. Hypertrophic cells rely more on the mitochondrial respiration than the resting cells.
Elsevier
