TA-cloning vectors for rapid and cheap cloning of zebrafish transgenesis constructs
LB Miles, H Verkade - Zebrafish, 2014 - research.monash.edu
LB Miles, H Verkade
Zebrafish, 2014•research.monash.eduHere we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-
TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and
final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning
expression vector, pCS2+ TA, for rapid generation of new expression plasmids.
TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and
final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning
expression vector, pCS2+ TA, for rapid generation of new expression plasmids.
Abstract
Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning expression vector, pCS2+ TA, for rapid generation of new expression plasmids.
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